| Expression pattern: |
DN |
| Associated gene: |
PCNA, Cleaved-caspase 3, MMP, MMP-9 |
| Associated microRNA: |
miR-942 |
| Biological function: |
CircFBXW7 suppresses ccRCC cell proliferation, migration and invasion, induces apoptosis, and mediates the anticancer effects of propofol on ccRCC tumorigenesis. |
| Molecular mechanism: |
Propofol upregulates circFBXW7, and circFBXW7 directly binds to and suppresses miR-942, thereby inhibiting malignant phenotypes of ccRCC cells. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); metastasis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
Lower circFBXW7 expression was correlated with tumor size, clinical stage, lymph node metastasis, and distant metastasis; the authors proposed circFBXW7 as a stable biomarker for ccRCC combined treatment with propofol. |
| Description: |
In ccRCC, circFBXW7 is down-regulated in tumor tissues and RCC cell lines, while propofol treatment increases its expression. CircFBXW7 acts as a tumor suppressor by directly targeting miR-942, inhibiting proliferation, migration and invasion and promoting apoptosis; this axis contributes to the anticancer effect of propofol in vitro and in a xenograft model. |
| Confidence score: |
0.7294 |