circRNA basic information
circBase ID: -
Name: -
Synonym: hsa_circ_100338
Host Gene: ZEB1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue
Cell lines:

HepG2; HuH‐7; HL‐7702
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ZEB1, AGO2
Associated microRNA: miR‐141‐3p
Biological function: promotes proliferation
Molecular mechanism: ceRNA (miRNA sponge) mechanism: circ-100338 sponges miR-141-3p to upregulate ZEB1
Biological pathway or process:

proliferation (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

RT-qPCR; RNase R Treatment; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Bioinformatics Analysis; Microarray
Clinical significance:

-
Description:

circ-100338 is up-regulated in HCC cell lines and mainly localizes to the cytoplasm. It promotes HCC cell proliferation by acting as a ceRNA that sponges miR-141-3p, thereby increasing ZEB1 expression; piplartine suppresses proliferation partly by reducing circ-100338.
Confidence score:

0.7893
Other information
Title:

Piplartine attenuates the proliferation of hepatocellular carcinoma cells via regulating hsa_circ_100338 expression.

Journal: Cancer medicine
Published: 2020
PubMed ID: 32281302
Study type:

combined biological and clinical study
Data availability: All data used in this study are available from the corresponding author upon reasonable request.
Code availability: -