| Expression pattern: |
UP |
| Associated gene: |
Sam68, METTL3 promoter, METTL3, H3K27ac, Ki-67 |
| Associated microRNA: |
- |
| Biological function: |
Promotes EBVaGC cell proliferation, migration, invasion, tumor growth and lung metastasis, and inhibits apoptosis; associated with distant metastasis and poor prognosis. |
| Molecular mechanism: |
ebv-circRPMS1 binds the RNA-binding protein Sam68 and recruits/facilitates Sam68 at the METTL3 promoter, inducing METTL3 transcription, increasing RNA m6A levels, and promoting EBVaGC progression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes); m6A modification (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; FISH / smFISH; ISH (In Situ Hybridization); IHC (Immunohistochemistry); Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; ChIP / ChIP-seq; Luciferase Reporter Assay; MeRIP / MeRIP-seq; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; H&E Staining; ELISA; Western Blot; Cohort Study; Survival Analysis; ROC Analysis |
| Clinical significance: |
High ebv-circRPMS1 expression was associated with distant metastasis and predicted shorter overall survival/poor prognosis in EBVaGC patients. |
| Description: |
ebv-circRPMS1 is an EBV-encoded circRNA upregulated in EBV-associated gastric carcinoma. It functions as an oncogenic circRNA by promoting proliferation, migration, invasion, tumor growth and metastasis while inhibiting apoptosis. Mechanistically, it binds Sam68 and promotes Sam68-dependent METTL3 transcription, increasing m6A-related activity; clinically, high ebv-circRPMS1 expression is linked to distant metastasis and poor overall survival. |
| Confidence score: |
0.8628 |