| Expression pattern: |
UP |
| Associated gene: |
Ifnb1 mRNA, ISRE promoter, IAV M, IAV M1 |
| Associated microRNA: |
hsa-miR-3945, hsa-miR-4686, hsa-miR-548 CE-3p, hsa-miR-5683, hsa-miR-6071 |
| Biological function: |
circRNA_0082633 suppresses IAV infection and viral proliferation, attenuates IAV replication, and enhances IAV-triggered host type I IFN response. |
| Molecular mechanism: |
circRNA_0082633 is induced by IAV infection through type I IFN-associated JAK-STAT signaling and enhances type I IFN signaling through ISRE promoter activity and Ifnb1 mRNA levels, forming a positive feedback loop that contributes to antiviral innate immune response. |
| Biological pathway or process: |
viral replication (inhibits); STING/IFN (promotes); JAK/STAT (other); immune regulation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Bioinformatics Analysis; Transfection; Western Blot; Luciferase Reporter Assay |
| Clinical significance: |
- |
| Description: |
circRNA_0082633 is a human ZC3HAV1-derived circRNA up-regulated in A549 cells after IAV/PR8 infection. Functionally, it acts as an anti-IAV host factor by suppressing viral replication and enhancing type I IFN signaling, while its expression is at least partly induced through JAK-STAT signaling. The study also predicted potential circ_0082633-miRNA interactions, but the main experimentally supported mechanism centers on type I IFN/JAK-STAT-related antiviral response. |
| Confidence score: |
0.5495 |