| Expression pattern: |
UP |
| Associated gene: |
IL-6, TNF-alpha, ERK/NF-kappaB pathway, IkappaB, NF-kappaB p65, RAGE |
| Associated microRNA: |
hsa-miR-149-5p |
| Biological function: |
Promotes macrophage inflammatory response, enhances IL-6 and TNF-alpha expression and secretion, and promotes vascular smooth muscle cell apoptosis in a macrophage-VSMC coculture system. |
| Molecular mechanism: |
hsa_circ_0087352 acts as an endogenous hsa-miR-149-5p sponge to promote IL-6 transcription and inflammatory cytokine secretion, thereby expanding ERK/NF-kappaB signaling and promoting NF-kappaB p65 phosphorylation and nuclear translocation. |
| Biological pathway or process: |
inflammation (promotes); immune regulation (promotes); NF-kappaB (promotes); ERK (promotes); apoptosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNA-seq; RNase R Treatment; FISH / smFISH; IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; Western Blot; ELISA; Annexin V/PI Flow Cytometry; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0087352 can be used as a new biomarker and therapeutic target for abdominal aortic aneurysm. |
| Description: |
hsa_circ_0087352 is up-regulated in human AAA specimens and LPS-stimulated macrophages. It promotes macrophage inflammatory responses by sponging hsa-miR-149-5p, increasing IL-6 and TNF-alpha production and activating ERK/NF-kappaB signaling. Its overexpression in macrophages also promotes VSMC apoptosis, supporting its proposed value as a biomarker and therapeutic target for AAA. |
| Confidence score: |
0.7924 |