circRNA basic information
circBase ID: hsa_circ_0060467
Name: hsa_circ_MYBL2
Synonym: circMYBL2
Host Gene: MYBL2
Genomic location(hg19): chr20:42338602-42345122:+
Genomic location(hg38): chr20:43709962-43716482:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BCLM tissues; primary BC tissues
Cell lines:

MCF-7; BT-474; MDA-MB-468; HCC38; MDA-MB-231; BT-549; SKBR-3; MCF-10A; Hs 578Bst
In vivo animal model:

cell line-derived xenograft; other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: E2F1, eIF4A3
Associated microRNA: miR-1205
Biological function: promotes proliferation; promotes migration; promotes tumor growth; promotes liver metastasis; promotes EMT
Molecular mechanism: sponges miR-1205 to counteract miR-1205-mediated inhibition of E2F1; recruits eIF4A3 and stabilizes E2F1 mRNA; increases E2F1 to facilitate EMT
Biological pathway or process:

proliferation (promotes); migration (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes); mRNA stability (promotes)
Detected method:
Q
M
Validation methods:

Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Co-IP
Clinical significance:

potential therapeutic target for patients with BCLM; prognosis correlation with circMYBL2 not verified
Description:

circMYBL2 (hsa_circ_0060467) is up-regulated in BCLM tissues and BC cells and promotes BC proliferation, migration, tumor growth, and liver metastasis. It sponges miR-1205 and recruits eIF4A3 to increase E2F1 (including via stabilizing E2F1 mRNA), thereby promoting EMT.
Confidence score:

0.8266
Other information
Title:

Hsa_circ_0060467 promotes breast cancer liver metastasis by complexing with eIF4A3 and sponging miR-1205.

Journal: Cell death discovery
Published: 2023
PubMed ID: 37160894
Study type:

combined biological and clinical study
Data availability: The data supporting the findings of this study are available within the article and its supplementary materials.
Code availability: -