circRNA basic information
circBase ID: -
Name: -
Synonym: circRNA_100876
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; normal tissues; normal adjacent tissues
Cell lines:

LoVo; SW620; SW480; Caco-2; HT-29; HCT-8; FHC; 293 T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: Bcl-2, cleaved caspase-3, Bax, E-cadherin, N-cadherin, vimentin, Snail
Associated microRNA: miR-516b
Biological function: Promotes CRC cell proliferation, migration, invasion, metastasis, and EMT; inhibits apoptosis.
Molecular mechanism: circRNA_100876 sponges miR-516b; its silencing suppresses proliferation and metastasis, increases apoptosis through the caspase-dependent apoptotic pathway, and inhibits migration and invasion by interfering with the EMT signaling pathway.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Luciferase Reporter Assay; Western Blot; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circRNA_100876 expression is associated with poor clinical outcome, shorter overall survival, shorter disease-free survival, tumor differentiation, vascular invasion, tumor size, T stage, lymphatic metastasis, and distant metastasis; it may be a diagnostic and therapeutic biomarker for CRC.
Description:

circRNA_100876 is upregulated in colorectal cancer tissues and cell lines, and high expression is associated with worse clinicopathological features and poor survival. Functionally, circRNA_100876 promotes CRC proliferation, migration, invasion, metastasis and EMT while suppressing apoptosis, at least partly by targeting miR-516b.
Confidence score:

0.7078
Other information
Title:

Dysregulated circRNA_100876 contributes to proliferation and metastasis of colorectal cancer by targeting microRNA-516b (miR-516b).

Journal: Cancer biology & therapy
Published: 2020
PubMed ID: 32564659
Study type:

combined biological and clinical study
Data availability: -
Code availability: -