| Expression pattern: |
UP |
| Associated gene: |
IRF4 |
| Associated microRNA: |
miR-193a-3p |
| Biological function: |
Circ_0020123 promotes NSCLC cell proliferation, migration, glycolysis and tumor growth, inhibits apoptosis and autophagy, and contributes to malignant behaviors. |
| Molecular mechanism: |
Circ_0020123 acts as a ceRNA sponge for miR-193a-3p, thereby relieving miR-193a-3p-mediated suppression of IRF4 and upregulating IRF4 expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); apoptosis (inhibits); autophagy (inhibits); glycolysis (promotes); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0020123 expression is associated with poor prognosis and may be an underlying prognostic biomarker for NSCLC. |
| Description: |
Circ_0020123 is up-regulated in NSCLC tissues and cells, and high expression is associated with poor overall survival. Functionally, circ_0020123 promotes NSCLC tumor growth, proliferation, migration, and glycolysis while inhibiting apoptosis and autophagy. Mechanistically, it sponges miR-193a-3p to increase IRF4 expression, forming a circ_0020123/miR-193a-3p/IRF4 regulatory axis. |
| Confidence score: |
0.8438 |