| Expression pattern: |
UP |
| Associated gene: |
HuR, BIRC3 |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation, migration, invasion, tumor growth and metastasis in HCC |
| Molecular mechanism: |
circNUP54 binds HuR and promotes its cytoplasmic export/accumulation, enhancing HuR-mediated stabilization of BIRC3 mRNA and activating NF-kappaB signaling |
| Biological pathway or process: |
NF-kappaB (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; divergent primers PCR; Clinical Sample Validation; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); IF (Immunofluorescence); Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; RNA-seq; Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
circNUP54 overexpression was correlated with aggressive cancerous behaviors and poor outcomes; high levels of circNUP54 contributed to unfavorable overall survival (OS) and recurrence-free survival (RFS) |
| Description: |
circNUP54 (hsa_circ_0070039), derived from NUP54, is up-regulated in HCC and predicts poor prognosis. It promotes HCC proliferation, migration/invasion and tumor growth/metastasis by binding HuR, enhancing HuR cytoplasmic export and stabilizing BIRC3 mRNA, which activates NF-kappaB signaling. |
| Confidence score: |
0.8854 |