| Expression pattern: |
UP |
| Associated gene: |
RUNX1, ANXA11, AGO2 |
| Associated microRNA: |
miR-485-5p |
| Biological function: |
Promotes proliferation, migration and invasion, and inhibits apoptosis of human NF1-associated MPNST cells; functions as an oncogene in NF1-associated MPNST cells. |
| Molecular mechanism: |
circ_0061,587 acts as a ceRNA that sequesters miR-485-5p to up-regulate RUNX1 and ANXA11 expression, thereby promoting malignant behaviors of NF1-associated MPNST cells. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_0061,587 might be a potential diagnostic and prognostic marker or biomarker for NF1. |
| Description: |
circ_0061,587 is up-regulated in human NF1-associated MPNST cell lines and acts as an oncogenic circRNA. It promotes proliferation, migration and invasion while suppressing apoptosis by sponging miR-485-5p and increasing RUNX1 and ANXA11 expression. The study suggests circ_0061,587 may serve as a potential biomarker for NF1. |
| Confidence score: |
0.809 |