| Expression pattern: |
UP |
| Associated gene: |
SND1, MYB, BUB1, CPC |
| Associated microRNA: |
- |
| Biological function: |
Promotes docetaxel (DTX) resistance in prostate cancer cells; reduces DTX-induced apoptosis; supports error-free mitosis via mitosis error correction. |
| Molecular mechanism: |
circ_0004087 forms a circRNA-protein complex with SND1, enhancing SND1-mediated MYB transactivation, increasing BUB1 expression; BUB1 promotes CPC recruitment to centromeres to strengthen mitosis error correction, leading to DTX chemoresistance. |
| Biological pathway or process: |
chemoresistance (promotes); apoptosis (inhibits); cell cycle (promotes); other pathway/process (promotes) |
| Detected method: |
Q
S
M
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Agarose Gel Electrophoresis; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; Luciferase Reporter Assay; Transfection; Annexin V/PI Flow Cytometry; In Vivo Animal Model; TUNEL; RNA-seq; Western Blot; Mass Spectrometry; Microarray |
| Clinical significance: |
circ_0004087 may serve as a valuable prognostic biomarker and a potential therapeutic target in DTX-resistant PCa patients. |
| Description: |
circ_0004087 is upregulated in prostate cancer and promotes docetaxel resistance. It localizes mainly in the nucleus and binds SND1, enhancing SND1-mediated MYB transactivation to increase BUB1 expression, which strengthens CPC-dependent mitosis error correction and reduces DTX-induced apoptosis. |
| Confidence score: |
0.8293 |