| Expression pattern: |
DN |
| Associated gene: |
MAPK9, AGO2 |
| Associated microRNA: |
miR-421 |
| Biological function: |
Overexpression of circZNF720 inhibits HCC cell proliferation, colony formation, migration and invasion, and promotes apoptosis; circZNF720 acts as a tumor suppressor in HCC. |
| Molecular mechanism: |
circZNF720 functions through a ceRNA mechanism by sponging miR-421, thereby relieving miR-421-mediated repression of MAPK9 and increasing MAPK9 expression. |
| Biological pathway or process: |
ceRNA regulation (other); MAPK (promotes); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); metastasis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Low circ-ZNF720 expression is associated with worse prognosis; circZNF720 might be a useful therapeutic target for HCC. |
| Description: |
circZNF720 is downregulated in HCC tissues and cell lines, and low expression is associated with worse prognosis. Functionally, circZNF720 overexpression suppresses HCC cell proliferation, migration and invasion while promoting apoptosis. Mechanistically, circZNF720 acts as a cytoplasmic ceRNA that sponges miR-421 and increases MAPK9 expression, thereby inhibiting HCC progression. |
| Confidence score: |
0.817 |