circRNA basic information
circBase ID: hsa_circ_0016760
Name: hsa_circ_SNAP47
Synonym: circ_0016760
Host Gene: SNAP47
Genomic location(hg19): chr1:227935392-227947186:+
Genomic location(hg38): chr1:227747691-227759485:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: Non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissue specimens; matched normal tissue specimens; healthy lung tissues at the edge of the lung tumor; xenograft tumors
Cell lines:

BEAS-2B; A549; H1975; H1299; H460
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: NOVA2, AGO2, SNAP47 mRNA
Associated microRNA: miR-876-3p
Biological function: Promotes NSCLC cell proliferation, clonogenicity, migration, invasion, EMT, metastasis and tumor growth; inhibits apoptosis.
Molecular mechanism: circ_0016760 acts as a sponge of miR-876-3p to increase NOVA2 expression, thereby regulating growth and EMT of NSCLC cells.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circ_0016760 expression was related to poor overall survival in NSCLC patients and may be a potent therapeutic target for NSCLC.
Description:

circ_0016760 is upregulated in NSCLC tissues and cell lines, and high expression is associated with worse overall survival. Functionally, circ_0016760 promotes malignant NSCLC phenotypes including proliferation, migration, invasion, EMT, metastasis and in vivo tumor growth while suppressing apoptosis. Mechanistically, it sponges miR-876-3p to increase NOVA2 expression.
Confidence score:

0.8174
Other information
Title:

Circ_0016760 Serves as a Cancer Promoter in Non-small Cell Lung Cancer Through miR-876-3p/NOVA2 Axis.

Journal: Biochemical genetics
Published: 2022
PubMed ID: 35239092
Study type:

combined biological and clinical study
Data availability: All data generated or analyzed during this study are included in this article; supplementary material available at https:// doi. org/ 10. 1007/ s10528-022-10198-4
Code availability: -