| Expression pattern: |
UP |
| Associated gene: |
MAML3, AGO2 |
| Associated microRNA: |
miR-411-5p |
| Biological function: |
Promotes gastric cancer cell proliferation in vitro and in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-411-5p, thereby increasing MAML3 expression. |
| Biological pathway or process: |
proliferation (promotes); ceRNA regulation (promotes); Notch (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Northern Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Western Blot; Clinical Sample Validation; In Vivo Animal Model |
| Clinical significance: |
A promising biomarker for gastric cancer diagnosis and prognosis, and a potential therapeutic target. |
| Description: |
hsa_circ_0007967 is up-regulated in gastric cancer tissues and cell lines. It mainly localizes to the cytoplasm and promotes gastric cancer cell proliferation in vitro and in a cell line-derived xenograft model by sponging miR-411-5p, thereby increasing MAML3 expression (a Notch-related transcriptional co-activator). |
| Confidence score: |
0.8339 |