circRNA basic information
circBase ID: hsa_circ_0073181
Name: hsa_circ_RASGRF2
Synonym: circ_0073181
Host Gene: RASGRF2
Genomic location(hg19): chr5:80338695-80409739:+
Genomic location(hg38): chr5:81042876-81113920:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tumor tissue samples; matched adjacent normal tissue samples; tumor tissues
Cell lines:

THLE-2; Huh7; SNU-387; HUVEC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PTPRE, RASGRF2, Ago2, PCNA, Bax, Ki67, ERK1/2, JNK, p38
Associated microRNA: miR-548p
Biological function: circ_0073181 promotes HCC cell proliferation, migration, invasion, angiogenesis, metastasis and tumor growth, and inhibits apoptosis.
Molecular mechanism: circ_0073181 functions as a ceRNA/miRNA sponge for miR-548p to regulate PTPRE expression and promote HCC progression; circ_0073181 may also activate ERK1/2, JNK and p38 signaling.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); metastasis (promotes); ceRNA regulation (promotes); ERK (promotes); MAPK (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; Tube Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

circ_0073181 was highly expressed especially in advanced HCC tumor stages and might be a potential biomarker and therapeutic target.
Description:

circ_0073181 is upregulated in HCC tissues and cell lines and is associated with advanced tumor stage. Functional experiments indicate that circ_0073181 promotes HCC proliferation, migration, invasion, angiogenesis, metastasis and xenograft tumor growth while suppressing apoptosis. Mechanistically, circ_0073181 sponges miR-548p to regulate PTPRE expression, and may contribute to HCC progression partly through ERK1/2, JNK and p38 signaling activation.
Confidence score:

0.8299
Other information
Title:

Circular RNA circ_0073181 contributes to tumorigenesis by regulating protein tyrosine phosphatase receptor type E via miR-548p in hepatocellular carcinoma.

Journal: Anti-cancer drugs
Published: 2022
PubMed ID: 34657099
Study type:

combined biological and clinical study
Data availability: Please contact the correspondence author for the data request.
Code availability: -