| Expression pattern: |
UP |
| Associated gene: |
Sp1, Ago2 |
| Associated microRNA: |
miR-527 |
| Biological function: |
promotes GC cell proliferation, migration, invasion, tumor growth and metastasis; promotes EMT |
| Molecular mechanism: |
circ_0005529 sponges miR-527 to upregulate Sp1, thereby promoting proliferation, migration and EMT in gastric cancer |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining |
| Clinical significance: |
GC patients with high circ_0005529 expression had a relatively lower survival rate and were associated with poor differentiation, TNM stage and invasion tendency |
| Description: |
circ_0005529 (from VPS33B) is upregulated in gastric cancer and mainly localized in the cytoplasm. It promotes GC cell proliferation, migration/invasion and in vivo tumor growth/metastasis by sponging miR-527 and relieving repression of Sp1, thereby facilitating EMT-related phenotypes. |
| Confidence score: |
0.8457 |