circRNA basic information
circBase ID: hsa_circ_0061713
Name: hsa_circ_BRWD1
Synonym: circBRWD1
Host Gene: BRWD1
Genomic location(hg19): chr21:40568423-40601362:-
Genomic location(hg38): chr21:39196497-39229436:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

liver tumor tissues; adjacent non-tumorous tissues; tumor tissues
Cell lines:

HepG2.2.15; Hep3B; HepG2; Huh7
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: TNPO1, Ago2, HBx
Associated microRNA: miR-513a-5p
Biological function: Promotes HBV replication, cccDNA accumulation, HCC cell proliferation, colony formation, migration, invasion, and tumor growth; inhibits apoptosis.
Molecular mechanism: circBRWD1 acts as a ceRNA sponge for miR-513a-5p, upregulates TNPO1, and thereby facilitates HBV replication and HCC progression.
Biological pathway or process:

viral replication (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (other); EMT (promotes)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; IF (Immunofluorescence); ELISA; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

Targeting circBRWD1 may offer a novel therapeutic strategy for HBV-related HCC and potentially address HBV persistence.
Description:

circBRWD1 is overexpressed in HBV-positive HCC tissues and cells and functions as an oncogenic circRNA. It promotes HBV replication, cccDNA accumulation, tumor cell proliferation, migration, invasion, and in vivo tumor growth while suppressing apoptosis. Mechanistically, circBRWD1 sponges miR-513a-5p to upregulate TNPO1, forming a circBRWD1/miR-513a-5p/TNPO1 regulatory axis.
Confidence score:

0.869
Other information
Title:

CircBRWD1 promotes hepatitis B virus replication and hepatocellular carcinoma progression by regulating the miR-513a-5p/TNPO1 axis.

Journal: Experimental cell research
Published: 2025
PubMed ID: 40216011
Study type:

combined biological and clinical study
Data availability: GSE181988; available from the corresponding author upon reasonable request; Data will be made available on request
Code availability: -