circRNA basic information
circBase ID: hsa_circ_0013290
Name: -
Synonym: circ_0013290
Host Gene: -
Genomic location(hg19): chr1:100335955-100343384:+
Genomic location(hg38): chr1:99870399-99877828:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; adjacent healthy tissues; adjacent normal liver tissues; primary tumors from Huh-7 cells

Cell lines:

Hep3B; Huh-7; THLE-2; HUVECs

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: MMP16, CyclinD1, N-cadherin
Associated microRNA: miR-139-5p
Biological function: circ_0013290 promotes HCC cell malignancy, including cell viability, proliferation, invasion, tube formation and tumor tumorigenesis, and inhibits apoptosis; Matrine suppresses HCC malignancy partly by downregulating circ_0013290.
Molecular mechanism: circ_0013290 acts as a miR-139-5p sponge to regulate MMP16 expression; Matrine inhibits HCC progression by decreasing circ_0013290 and subsequently affecting the miR-139-5p/MMP16 pathway.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); invasion (promotes); angiogenesis (promotes); ceRNA regulation (other); other pathway/process (other)

Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)

Clinical significance:

-

Description:

circ_0013290 is up-regulated in HCC tissues and cell lines and functions as a cancer-promoting circRNA. It sponges miR-139-5p to increase MMP16 expression, thereby promoting HCC cell proliferation, invasion, tube formation and tumorigenesis while suppressing apoptosis. Matrine exerts anti-HCC effects by downregulating circ_0013290 and modulating the circ_0013290/miR-139-5p/MMP16 pathway.

Confidence score:

0.8006

Other information
Title:

Matrine inhibits hepatocellular carcinoma cell malignancy through the circ_0013290/miR-139-5p/MMP16 pathway.

Journal: Histology and histopathology
Published: 2023
PubMed ID: 36594718
Study type:

combined biological and clinical study

Data availability: TCGA dataset
Code availability: -