circRNA basic information
circBase ID: hsa_circ_0020123
Name: hsa_circ_PDZD8
Synonym: circ_PDZD8
Host Gene: PDZD8
Genomic location(hg19): chr10:119042605-119049859:-
Genomic location(hg38): chr10:117283094-117290348:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008903
MONDO name: lung cancer
Disease details: lung cancer
Disease DO ID:
 1324
Disease MeSH ID:
 -
Disease NCIt ID:
 C7377
Disease ICD11 ID:
 -
Disease OMIM ID:
 211980
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues derived from xenograft; xenografts
Cell lines:

A549; PC9; HBE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: GOLT1A, PCNA, Ki-67
Associated microRNA: miR-516b-5p
Biological function: circ_PDZD8 promotes lung carcinoma cell growth ability, proliferation, migration, invasion, metastasis and tumor growth, and inhibits apoptosis; its upregulation attenuates lidocaine-induced anti-tumor effects.
Molecular mechanism: circ_PDZD8 acts as a ceRNA sponge for miR-516b-5p to regulate GOLT1A expression, thereby modulating lidocaine-mediated lung carcinoma malignant behaviors.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); ceRNA regulation (promotes); drug resistance (promotes)
Detected method:
Q
Validation methods:

Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis
Clinical significance:

potential attractive biomarker
Description:

circ_PDZD8 is up-regulated in lung carcinoma cell lines and is reduced by lidocaine treatment. Functionally, circ_PDZD8 counteracts lidocaine-induced suppression of proliferation, migration, invasion and xenograft growth, while reducing apoptosis. Mechanistically, it sponges miR-516b-5p to increase GOLT1A expression, forming the circ_PDZD8/miR-516b-5p/GOLT1A axis.
Confidence score:

0.786
Other information
Title:

Lidocaine represses the malignant behavior of lung carcinoma cells via the circ_PDZD8/miR-516b-5p/GOLT1A axis.

Journal: Histology and histopathology
Published: 2022
PubMed ID: 35060113
Study type:

biological research
Data availability: -
Code availability: -