| Expression pattern: |
UP |
| Associated gene: |
SHMT2 |
| Associated microRNA: |
miR-449a |
| Biological function: |
Exosomal circ_0063526 promotes cisplatin resistance in gastric cancer cells, enhances migration, invasion and autophagy, and its knockdown increases CDDP sensitivity. |
| Molecular mechanism: |
Exosome-mediated transfer; ceRNA mechanism in which circ_0063526 sponges miR-449a to upregulate SHMT2 expression, thereby enhancing cisplatin resistance. |
| Biological pathway or process: |
chemoresistance (promotes); drug resistance (promotes); migration (promotes); invasion (promotes); autophagy (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; Bioinformatics Analysis |
| Clinical significance: |
High serum exosomal circ_0063526 is associated with poor response to CDDP treatment and may serve as a promising diagnostic biomarker for gastric cancer patients. |
| Description: |
circ_0063526 is up-regulated in gastric cancer tissues, cells, CDDP-resistant cells, and serum exosomes from CDDP-resistant patients. It can be transferred via exosomes to CDDP-sensitive gastric cancer cells and promotes cisplatin resistance, migration, invasion, and autophagy by sponging miR-449a and increasing SHMT2 expression. Serum exosomal circ_0063526 is associated with poor response to cisplatin treatment and may have diagnostic biomarker value. |
| Confidence score: |
0.7032 |