circRNA basic information
circBase ID: hsa_circ_0006790
Name: hsa_circ_MEMO1
Synonym: circ_6790 / circMEMO1
Host Gene: MEMO1
Genomic location(hg19): chr2:32142994-32145979:-
Genomic location(hg38): chr2:31917925-31920910:-
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005184
MONDO name: pancreatic ductal adenocarcinoma
Disease details: pancreatic ductal adenocarcinoma / PDAC
Disease DO ID:
 3498, 3587
Disease MeSH ID:
 D021441
Disease NCIt ID:
 C9120
Disease ICD11 ID:
 581089833
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

xenograft tumors; liver; lung; blood; urine
Cell lines:

PANC-1; CFPAC-1; hTERT-HPNE; Human BM-MSC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: CBX7, S100A11, DNMT1, DNMT3A, DNMT3B
Associated microRNA: -
Biological function: Suppresses PDAC cell growth, proliferation, clonogenic ability, migration, invasion, metastasis, immune escape and immunosuppression, and promotes apoptosis and T cell-mediated tumor killing.
Molecular mechanism: BM-MSC-derived exosomal circ_6790 binds CBX7 and promotes CBX7 nuclear translocation; CBX7 recruits DNMT1, DNMT3A and DNMT3B to the S100A11 promoter, increases S100A11 DNA methylation, represses S100A11 transcription, and thereby reduces PDAC growth, metastasis and immune escape.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); apoptosis (promotes); immune regulation (promotes); other pathway/process (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Co-IP; ChIP / ChIP-seq; MSP (Methylation-Specific PCR); Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; ELISA; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis
Clinical significance:

Exo from BM-MSC with upregulation of circ_6790 could be a potential therapy for PDAC.
Description:

This study identifies hsa_circ_0006790/circ_6790 as a BM-MSC-derived exosomal circRNA that is reduced in PDAC cells compared with normal pancreatic ductal cells but increased in PDAC cells after Exo treatment. Exosomal circ_6790 binds CBX7, promotes CBX7 nuclear translocation, increases DNMT-mediated methylation of the S100A11 promoter, represses S100A11 transcription, and thereby inhibits PDAC growth, metastasis and immune escape.
Confidence score:

0.8179
Other information
Title:

Circ_0006790 carried by bone marrow mesenchymal stem cell-derived exosomes regulates S100A11 DNA methylation through binding to CBX7 in pancreatic ductal adenocarcinoma.

Journal: American journal of cancer research
Published: 2022
PubMed ID: 35693076
Study type:

combined biological and bioinformatics study
Data availability: Circular RNA Interactome; exoR-Base 2.0; StarBase; RBP suite; GEPIA; TIMER; UALCAN; Cistrome Data Browser
Code availability: -