| Expression pattern: |
UP |
| Associated gene: |
SLC7A11, Ago2, E-cadherin, vimentin, KI67 |
| Associated microRNA: |
miR-520a-5p |
| Biological function: |
circFOXP1 promotes lung cancer cell viability, colony formation, proliferation, migration, invasion, epithelial-to-mesenchymal transition and tumor growth, while repressing ferroptosis. |
| Molecular mechanism: |
circFOXP1 acts as a competing endogenous RNA that directly sponges miR-520a-5p, thereby increasing SLC7A11 expression and suppressing ferroptosis in lung cancer cells. |
| Biological pathway or process: |
ferroptosis (inhibits); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circFOXP1 is a potential diagnostic biomarker, and its up-regulation is closely correlated with poor overall survival of lung cancer patients. |
| Description: |
circFOXP1 is up-regulated in lung cancer tissues and high expression is associated with poor overall survival. Functionally, circFOXP1 promotes lung cancer cell proliferation, migration, invasion, EMT and xenograft tumor growth while inhibiting ferroptosis. Mechanistically, it sponges miR-520a-5p to increase SLC7A11 expression, supporting malignant progression and suggesting potential diagnostic and therapeutic relevance. |
| Confidence score: |
0.8769 |