circRNA basic information
circBase ID: hsa_circ_0018103
Name: hsa_circ_ARHGAP12
Synonym: circARHGAP12
Host Gene: ARHGAP12
Genomic location(hg19): chr10:32150322-32199491:-
Genomic location(hg38): chr10:31861394-31910563:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005015
MONDO name: diabetes mellitus
Disease details: diabetic wound
Disease DO ID:
 9351
Disease MeSH ID:
 D003920
Disease NCIt ID:
 C2985
Disease ICD11 ID:
 465177735
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

subcutaneous adipose tissue; skin wound tissues
Cell lines:

-
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
DN
Associated gene: ATG16L1, ULK2, AGO2, ATG5, Beclin1, p62, LC3B, Bim, Bcl-2, Bax, VEGF, fibroblast growth factor, TNF-a, IL-1b, IL-18
Associated microRNA: miR-301b-3p
Biological function: Induces MSC autophagy, suppresses high glucose-triggered MSC apoptosis, promotes MSC survival in diabetic wounds, accelerates wound healing, promotes revascularization and epithelialization, and modulates inflammatory cytokine secretion.
Molecular mechanism: circARHGAP12 acts as a cytoplasmic miRNA sponge for miR-301b-3p, thereby upregulating the miR-301b-3p target genes ATG16L1 and ULK2 and enhancing autophagy to protect MSCs from apoptosis under high glucose stress.
Biological pathway or process:

autophagy (promotes); apoptosis (inhibits); ceRNA regulation (promotes); angiogenesis (promotes); inflammation (inhibits); other pathway/process (promotes)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Annexin V/PI Flow Cytometry; TUNEL; Western Blot; In Vivo Animal Model; H&E Staining; ELISA; IF (Immunofluorescence); Bioinformatics Analysis
Clinical significance:

Potential therapeutic target for MSC-based diabetic wound healing.
Description:

circARHGAP12 is downregulated in human MSCs under high-glucose conditions relevant to diabetic wounds. It functions as a cytoplasmic ceRNA that sponges miR-301b-3p, increases ATG16L1 and ULK2 expression, enhances autophagy, reduces MSC apoptosis, and improves MSC-mediated repair of diabetic wounds in vivo.
Confidence score:

0.8131
Other information
Title:

CircARHGAP12 Triggers Mesenchymal Stromal Cell Autophagy to Facilitate its Effect on Repairing Diabetic Wounds by Sponging miR-301b-3p/ATG16L1 and miR-301b-3p/ULK2.

Journal: The Journal of investigative dermatology
Published: 2022
PubMed ID: 34933019
Study type:

biological research
Data availability: GSE168890
Code availability: -