| Expression pattern: |
UP |
| Associated gene: |
15 corresponding target mRNAs |
| Associated microRNA: |
miR-1282, miR-1243, miR-1178, miR-638, miR-451 |
| Biological function: |
promotes proliferation, invasion, and migration of gastric cancer cells |
| Molecular mechanism: |
Predicted miRNA sponge (ceRNA) mechanism: circ_0066444 may interact with 5 miRNAs and corresponding target mRNAs; not experimentally validated in this study. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; MTT; Transwell Assay; Wound Healing Assay; ROC Analysis |
| Clinical significance: |
Upregulated in GC tissues; positively associated with lymph node metastasis; ROC AUC 0.7328 with sensitivity 70.75% and specificity 68.87%; proposed as a novel biomarker for GC. |
| Description: |
circ_0066444 is upregulated in gastric cancer tissues and correlates with lymph node metastasis. Functionally, circ_0066444 promotes GC cell proliferation, migration, and invasion in vitro, and is proposed to act through a predicted circRNA-miRNA-mRNA (ceRNA) network. |
| Confidence score: |
0.6148 |