circRNA basic information
circBase ID: hsa_circ_0058147
Name: hsa_circ_FN1
Synonym: circFN1
Host Gene: FN1
Genomic location(hg19): chr2:216283964-216286966:-
Genomic location(hg38): chr2:215419241-215422243:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
10534
Disease MeSH ID:
-
Disease NCIt ID:
C9331
Disease ICD11 ID:
1397617262
Disease OMIM ID:
613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissues; CDDP-resistant GC tissues; CDDP-sensitive GC tissues

Cell lines:

SGC7901CDDP; BGC823CDDP; SGC7901; BGC823; 293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: caspase-3
Associated microRNA: miR-182-5p
Biological function: Promotes cisplatin resistance and GC cell viability, inhibits apoptosis, and is associated with aggressive biological behavior in GC patients treated with CDDP.
Molecular mechanism: circFN1 acts as a ceRNA by sponging miR-182-5p, thereby inhibiting miR-182-5p-mediated activation of apoptosis via the caspase-3 signaling pathway and facilitating CDDP resistance.
Biological pathway or process:

apoptosis (inhibits); proliferation (promotes); chemoresistance (promotes); drug resistance (promotes); ceRNA regulation (other)

Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; RNA-seq; FISH / smFISH; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; TUNEL; Western Blot; In Vivo Animal Model; Bioinformatics Analysis

Clinical significance:

CircFN1 upregulation in GC patients treated with CDDP was significantly correlated with aggressive biological behavior, CDDP resistance, clinical stage, and T classification; circFN1 is implicated as a therapeutic target for GC patients treated with CDDP.

Description:

circFN1 is up-regulated in cisplatin-resistant gastric cancer tissues and cell lines. It promotes CDDP resistance by acting as a ceRNA sponge for miR-182-5p, reducing apoptosis through the caspase-3 signaling pathway and enhancing cell viability and tumor growth under cisplatin treatment. Clinically, higher circFN1 expression is associated with CDDP resistance and more aggressive clinicopathological features.

Confidence score:

0.8206

Other information
Title:

A novel circular RNA circFN1 enhances cisplatin resistance in gastric cancer via sponging miR-182-5p.

Journal: Journal of cellular biochemistry
Published: 2020
PubMed ID: 31898357
Study type:

combined biological and clinical study

Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.
Code availability: -