| Expression pattern: |
DN |
| Associated gene: |
Ago2 |
| Associated microRNA: |
miR-198 |
| Biological function: |
Promotes trophoblast cell invasion and migration, inhibits oxidative stress, and blocks PE progression; decreased plasma circ_0004104 may serve as a biomarker for PE severity. |
| Molecular mechanism: |
circ_0004104 acts as a miR-198 sponge; miR-198 silencing counteracts the effects of circ_0004104 knockdown on trophoblast invasion, migration and oxidative stress. |
| Biological pathway or process: |
invasion (promotes); migration (promotes); ceRNA regulation (other); other pathway/process (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Transwell Assay; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Decreased plasma circ_0004104 could be a new biomarker for PE severity; plasma circ_0004104 distinguished severe PE from mild PE with AUC 0.835, increasing to 0.862 when combined with sFlt-1/PlGF ratio. |
| Description: |
circ_0004104 is downregulated in plasma and placental samples from PE patients, especially in severe PE. Functionally, circ_0004104 promotes trophoblast invasion and migration and suppresses oxidative stress by sponging miR-198, thereby restraining PE progression. Clinically, plasma circ_0004104 may help distinguish severe PE from mild PE, particularly when combined with the sFlt-1/PlGF ratio. |
| Confidence score: |
0.8244 |