circRNA basic information
circBase ID: hsa_circ_0003204
Name: hsa_circ_USP36
Synonym: circUSP36
Host Gene: USP36
Genomic location(hg19): chr17:76798405-76800060:-
Genomic location(hg38): chr17:78802323-78803978:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005311
MONDO name: atherosclerosis
Disease details: atherosclerosis
Disease DO ID:
 1936
Disease MeSH ID:
 D050197
Disease NCIt ID:
 C35768
Disease ICD11 ID:
 109367356
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

HUVECs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: ROCK2
Associated microRNA: miR-20a-5p
Biological function: aggravates ox-LDL-induced HUVEC injury and inflammatory responses; promotes cell cycle arrest, apoptosis and inflammation; inhibits migration and invasion (under ox-LDL)
Molecular mechanism: circUSP36 acts as a ceRNA/miRNA sponge for miR-20a-5p to regulate ROCK2 expression
Biological pathway or process:

apoptosis (promotes); cell cycle (promotes); migration (inhibits); invasion (inhibits); inflammation (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Transfection; CCK8; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; ELISA; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation)
Clinical significance:

-
Description:

circUSP36 is up-regulated in ox-LDL-treated HUVECs (an in vitro atherosclerosis model) and promotes ox-LDL-induced endothelial injury and inflammation. Functionally, circUSP36 knockdown reduces cell cycle arrest, apoptosis, and inflammatory cytokines and restores migration/invasion. Mechanistically, circUSP36 sponges miR-20a-5p to derepress ROCK2 (circUSP36/miR-20a-5p/ROCK2).
Confidence score:

0.5998
Other information
Title:

CircUSP36 knockdown alleviates oxidized low‑density lipoprotein‑induced cell injury and inflammatory responses in human umbilical vein endothelial cells via the miR‑20a‑5p/ROCK2 axis.

Journal: International journal of molecular medicine
Published: 2021
PubMed ID: 33576448
Study type:

biological research
Data availability: All data generated or analyzed during this study are included in this published article or are available from the corresponding author on reasonable request.
Code availability: -