circRNA basic information
circBase ID: -
Name: hsa_circ_ESYT2
Synonym: circEsyt2
Host Gene: Esyt2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004995
MONDO name: cardiovascular disorder
Disease details: cardiovascular diseases
Disease DO ID:
 1287
Disease MeSH ID:
 D002318
Disease NCIt ID:
 C2931
Disease ICD11 ID:
 424293435
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

aortae; atherosclerotic tissues; atherosclerotic plaques; human coronary arteries; carotid arteries
Cell lines:

HASMCs; HEK293T; mouse VSMCs
In vivo animal model:

genetically engineered animal model; other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: PCBP1, U2AF65, p53 pre-mRNA, p53beta, cyclin D1, p21, PUMA, NOXA
Associated microRNA: -
Biological function: circEsyt2 promotes VSMC proliferation, migration, phenotype switching/dedifferentiation, neointima formation and neointimal hyperplasia, and inhibits apoptosis and differentiation during vascular remodeling.
Molecular mechanism: circEsyt2 directly binds PCBP1 and promotes its cytoplasmic retention, thereby inhibiting PCBP1-dependent, U2AF65-mediated alternative splicing of p53 pre-mRNA to p53beta and altering p53 target genes involved in VSMC proliferation and apoptosis.
Biological pathway or process:

proliferation (promotes); migration (promotes); apoptosis (inhibits); cell cycle (promotes); p53 signaling (inhibits); mRNA stability (other); other pathway/process (other)
Detected method:
Q
H
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; TUNEL; In Vivo Animal Model; H&E Staining; Oil Red O Staining; Western Blot; Bioinformatics Analysis
Clinical significance:

circEsyt2 expression was much higher in arteries from patients with severe CAD than in patients with mild CAD, suggesting a proatherosclerotic role and potential therapeutic relevance in vascular remodeling-related diseases.
Description:

circEsyt2 is upregulated in atherosclerotic and injured vessels and in severe CAD arteries, and it promotes VSMC remodeling by enhancing proliferation and migration while suppressing apoptosis and differentiation. Mechanistically, circEsyt2 binds PCBP1 in the cytoplasm, limits PCBP1 nuclear translocation, suppresses PCBP1/U2AF65-dependent p53beta splicing, and thereby alters p53 target genes that control VSMC proliferation and apoptosis.
Confidence score:

0.8469
Other information
Title:

Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.

Journal: The Journal of clinical investigation
Published: 2021
PubMed ID: 34907911
Study type:

combined biological and clinical study
Data availability: GSE133269; GSE133270; iProx: IPX0001671000; Supplemental Table 1; Supplemental Table 2; Supplemental Table 3; Supplemental Table 4; Supplemental Table 5; Supplemental Table 6
Code availability: -