| Expression pattern: |
UP |
| Associated gene: |
vimentin, CCND3 |
| Associated microRNA: |
miR-138 |
| Biological function: |
circRBM23 functions as an oncogene in HCC; its upregulation increases HCC cell viability and migration, while downregulation decreases viability, proliferation, migration, blocks cell cycle progression, promotes miR-138 expression, and reduces tumor growth-related effects. |
| Molecular mechanism: |
circRBM23 regulates the miR-138-mediated signaling pathway, suppressing tumor suppressor miR-138 and restoring vimentin and CCND3 protein expression; bioinformatics predicted miR-138 binding sites in human circRBM23, consistent with a ceRNA-like miRNA sponge mechanism. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); cell cycle (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
The protumor role of circRBM23 indicated potential prognostic and therapeutic value in HCC. |
| Description: |
circRBM23 is up-regulated in HCC tissues and HCC cell lines and acts as an oncogenic circRNA. It promotes HCC cell viability, proliferation, cell cycle progression, migration, and xenograft tumor growth by regulating the tumor suppressor miR-138 and downstream vimentin and CCND3 expression. The authors suggest that circRBM23 may have prognostic and therapeutic value in HCC. |
| Confidence score: |
0.5761 |