circRNA basic information
circBase ID: hsa_circ_0000558
Name: hsa_circ_EIF4G3
Synonym: circ_000558 / hsa_circEIF4G3_054
Host Gene: EIF4G3
Genomic location(hg19): chr14:89628044-89629313:-
Genomic location(hg38): chr14:89161700-89162969:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005086
MONDO name: renal cell carcinoma
Disease details: renal cell carcinoma / RCC
Disease DO ID:
 4450
Disease MeSH ID:
 D002292
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

RCC tissues; matched nontumor tissues; tumor tissues
Cell lines:

ACHN; 786-O; 769-P; Caki-1; HK2
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ARL4C
Associated microRNA: miR-1225-5p
Biological function: Promotes RCC cell proliferation and inhibits apoptosis.
Molecular mechanism: Acts as a miRNA sponge/ceRNA to inhibit miR-1225-5p, thereby upregulating ARL4C.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); ceRNA regulation (other)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

-
Description:

circ_000558 is up-regulated in RCC and mainly localized in the cytoplasm. It promotes RCC cell proliferation and suppresses apoptosis by sponging miR-1225-5p, thereby increasing ARL4C expression and enhancing tumor growth in a xenograft model.
Confidence score:

0.6867
Other information
Title:

The Effects of circ_000558/miR-1225-5p/ARL4C on Regulating the Proliferation of Renal Cell Carcinoma Cells.

Journal: Journal of oncology
Published: 2023
PubMed ID: 36778920
Study type:

combined biological and bioinformatics study
Data availability: GSE100186; GSE23085; GSE16441; GSE100666
Code availability: -