circRNA basic information
circBase ID: -
Name: hsa_circ_NOTCH3
Synonym: circ_NOTCH3
Host Gene: NOTCH3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

liver cancer tissues; matched normal tissues; HCC cancer tissues; corresponding distal nontumor tissues; xenograft tumors

Cell lines:

LO2; HUH7; HepG2; 7721; LM3; HEK 293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: ZNF146
Associated microRNA: miR-875-5p
Biological function: Promotes HCC cell proliferation, colony formation, migration, invasion, tumor growth in vivo, and oxaliplatin resistance; inhibits apoptosis.
Molecular mechanism: circ_NOTCH3 functions as a miRNA sponge for miR-875-5p and regulates ZNF146 expression through the miR-875-5p/ZNF146 axis.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes); drug resistance (promotes); chemoresistance (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis

Clinical significance:

circ_NOTCH3 may be related to HCC pathogenesis, metastatic status, tumor burden, and may be a promising therapeutic target for HCC.

Description:

This study reports that circ_NOTCH3 is up-regulated in HCC tissues and cell lines and higher in metastatic HCC specimens. Functionally, circ_NOTCH3 promotes HCC cell growth, migration, invasion, tumor growth, and oxaliplatin resistance while suppressing apoptosis. Mechanistically, circ_NOTCH3 acts as a sponge for miR-875-5p to regulate ZNF146, forming a circ_NOTCH3/miR-875-5p/ZNF146 axis.

Confidence score:

0.8175

Other information
Title:

Hsa_circ_NOTCH3 regulates ZNF146 through sponge adsorption of miR-875-5p to promote tumorigenesis of hepatocellular carcinoma.

Journal: Journal of gastrointestinal oncology
Published: 2021
PubMed ID: 34790400
Study type:

combined biological and clinical study

Data availability: Available at https://dx.doi. org/10.21037/jgo-21-567
Code availability: -