| Expression pattern: |
UP |
| Associated gene: |
SERBP1 |
| Associated microRNA: |
miR-1258 |
| Biological function: |
Promotes HCC cell growth, proliferation, colony formation, cell cycle progression, migration, invasion, EMT progression, metastasis and tumor growth; inhibits apoptosis. |
| Molecular mechanism: |
circ_0046600 functions as a cytoplasmic ceRNA/miRNA sponge that competitively binds miR-1258, thereby up-regulating SERBP1; circ_0046600 can also be packaged into exosomes and released from HCC cells. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (other); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
circ_0046600 is up-regulated in HCC tissues and cells and acts as a tumor-promoting circRNA. It promotes HCC growth, metastasis and EMT while inhibiting apoptosis through a circ_0046600/miR-1258/SERBP1 ceRNA axis. The study also shows that circ_0046600 can be packaged into exosomes derived from HCC cells. |
| Confidence score: |
0.8124 |