| Expression pattern: |
UP |
| Associated gene: |
MED31 mRNA, MED31 |
| Associated microRNA: |
miR-548m |
| Biological function: |
circ-GLIS3 promotes TMZ-R glioma cell proliferation, invasion, migration, TMZ resistance and tumor growth, and inhibits cell cycle arrest and apoptosis. |
| Molecular mechanism: |
circ-GLIS3 acts as a miRNA sponge for miR-548m to positively regulate MED31 expression through the circ-GLIS3/miR-548m/MED31 axis. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); migration (promotes); apoptosis (inhibits); cell cycle (promotes); chemoresistance (promotes); drug resistance (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; Nuclear-Cytoplasmic Fractionation; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
circ-GLIS3 could serve as a potential therapeutic target in the treatment of glioma patients. |
| Description: |
circ-GLIS3 is upregulated in TMZ-resistant glioma cells and cell-derived exosomes. It promotes proliferation, invasion, migration, tumor growth and TMZ resistance while suppressing cell cycle arrest and apoptosis, mainly by sponging miR-548m to upregulate MED31 expression. |
| Confidence score: |
0.6746 |