circRNA basic information
circBase ID: hsa_circ_0011946
Name: hsa_circ_SCMH1
Synonym: circ-SCMH1
Host Gene: SCMH1
Genomic location(hg19): chr1:41578954-41618413:-
Genomic location(hg38): chr1:41113282-41152741:-
Subcellular localization: cytoplasm; extracellular vesicle
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004958
MONDO name: oral cavity squamous cell carcinoma
Disease details: oral squamous cell carcinoma
Disease DO ID:
 0050866
Disease MeSH ID:
 -
Disease NCIt ID:
 C4833
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues
Cell lines:

SCC-15; CAL-27; SCC-15/DDP; CAL-27/DDP; HOK
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: LIN28B, Ago2, extracellular vesicles (EVs)
Associated microRNA: miR-338-3p
Biological function: promotes cisplatin (DDP) chemoresistance; promotes colony formation; promotes migration and invasion; inhibits apoptosis; promotes tumor growth in vivo
Molecular mechanism: ceRNA mechanism: circ-SCMH1 sponges miR-338-3p to regulate LIN28B; transferred via extracellular vesicles from DDP-resistant cells to parental cells
Biological pathway or process:

chemoresistance (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); proliferation (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis; Clinical Sample Validation; Cohort Study
Clinical significance:

circ-SCMH1 expression was associated with TNM grade and lymph node metastasis of OSCC patients
Description:

circ-SCMH1 is upregulated in DDP-resistant OSCC tissues/cells and promotes cisplatin resistance and malignant phenotypes. It acts as a cytoplasmic ceRNA that sponges miR-338-3p to increase LIN28B, and it can be transferred as an extracellular vesicle cargo to elevate circ-SCMH1 in recipient parental cells.
Confidence score:

0.8324
Other information
Title:

Blocking circ-SCMH1 (hsa_circ_0011946) suppresses acquired DDP resistance of oral squamous cell carcinoma (OSCC) cells both in vitro and in vivo by sponging miR-338-3p and regulating LIN28B.

Journal: Cancer cell international
Published: 2021
PubMed ID: 34353342
Study type:

combined biological and clinical study
Data availability: The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
Code availability: -