circRNA basic information
circBase ID: -
Name: hsa_circ_GLS
Synonym: circRNA_22709
Host Gene: GLS
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; paired adjacent normal tissues; adjacent non-tumorous tissues
Cell lines:

BEAS-2B; A549; H1975; H460; H1299; PC-9; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: GSK3beta, beta-catenin, TCF1/LEF1, Axin2, Cyclin D1, PCNA, Bcl-2, Bax, cleaved caspase-9, cleaved caspase-3, DMT1, TFR1, FTH1, SLC7A11, GPX4, E-cadherin, N-cadherin, Vimentin, SNAI1
Associated microRNA: miR-410-3p
Biological function: circ_GLS inhibits proliferation, migration, invasion, tumor growth, stem-like properties, cell-cycle progression, beta-catenin pathway activation, and EMT, while promoting apoptosis and ferroptosis in NSCLC cells.
Molecular mechanism: circ_GLS acts through a ceRNA mechanism by directly binding miR-410-3p, thereby positively regulating GSK3beta and modulating the GSK3beta/beta-catenin pathway; this axis affects cell cycle, apoptosis, ferroptosis, and EMT.
Biological pathway or process:

Wnt/beta-catenin (inhibits); cell cycle (inhibits); apoptosis (promotes); ferroptosis (promotes); EMT (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (other)
Detected method:
Q
B
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; RT-qPCR; Northern Blot; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; TUNEL; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; Clinical Sample Validation; Survival Analysis; ROC Analysis; Bioinformatics Analysis
Clinical significance:

circ_GLS has good diagnostic efficacy in NSCLC tissues; high circ_GLS expression is associated with longer overall survival and lower death risk, and circ_GLS expression correlates with N stage, TNM stage, and differentiation.
Description:

circ_GLS is a GLS-derived human circRNA that is downregulated in NSCLC tumor tissues. It suppresses malignant NSCLC phenotypes by sponging miR-410-3p and positively regulating GSK3beta, thereby inhibiting beta-catenin signaling and EMT, restraining cell-cycle progression, and promoting apoptosis and ferroptosis. Clinically, higher circ_GLS expression is associated with better overall survival and shows diagnostic potential in NSCLC tissues.
Confidence score:

0.8868
Other information
Title:

Circ_GLS/miR-410-3p/GSK3beta/beta-catenin axis modulates malignant phenotypes of non-small cell lung cancer cells via regulation of cell cycle, apoptosis, ferroptosis, and EMT.

Journal: International journal of biological macromolecules
Published: 2025
PubMed ID: 41248794
Study type:

combined biological and clinical study
Data availability: TCGA database (https://portal.gdc.cancer.gov); Supplementary Materials; Supplementary Tables S1-S14; Supplementary Figures S1-S2; https://doi.org/10.1016/j.ijbiomac.2025.148830
Code availability: -