circRNA basic information
circBase ID: hsa_circ_0065217
Name: hsa_circ_SCAP
Synonym: hsa_circRNA_103349
Host Gene: SCAP
Genomic location(hg19): chr3:47468646-47470160:-
Genomic location(hg38): chr3:47427156-47428670:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005086
MONDO name: renal cell carcinoma
Disease details: renal cell carcinoma / RCC
Disease DO ID:
 4450
Disease MeSH ID:
 D002292
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

RCC tissues; adjacent non-cancerous tissues
Cell lines:

ACHN; SW839; OS-RC-2; A498; 786-O; Caki-1; HK-2
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: ALPK2, Ago2, SCAP, E-cadherin, vimentin
Associated microRNA: miR-214-3p
Biological function: Promotes RCC cell proliferation, colony formation, invasion, growth, metastasis, and malignant progression; silencing reduces RCC cell-line growth and aggressiveness.
Molecular mechanism: hsa_circ_0065217 acts as a ceRNA sponge for miR-214-3p and thereby promotes ALPK2 expression through the hsa_circ_0065217/miR-214-3p/ALPK2 axis.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); metastasis (promotes); EMT (promotes)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Microarray; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; IHC (Immunohistochemistry)
Clinical significance:

High hsa_circ_0065217 expression is linked to advanced TNM stages, larger tumor size, and lymph-node metastasis, and it is proposed as a potential therapeutic target for RCC.
Description:

hsa_circ_0065217 is up-regulated in RCC tissues and cell lines and is associated with advanced TNM stage, lymph-node metastasis, and larger tumor size. Functionally, it promotes RCC cell proliferation, colony formation, invasion, and metastatic phenotypes. Mechanistically, cytoplasmic hsa_circ_0065217 acts as a ceRNA for miR-214-3p to increase ALPK2 expression, forming a hsa_circ_0065217/miR-214-3p/ALPK2 axis that promotes malignant progression.
Confidence score:

0.8625
Other information
Title:

Hsa_circ_0065217 promotes growth and metastasis of renal cancer through regulating the miR-214-3p-ALPK2 axis.

Journal: Cell cycle (Georgetown, Tex.)
Published: 2021
PubMed ID: 34705617
Study type:

combined biological and clinical study
Data availability: GSE137836; GSE100186; TCGA
Code availability: -