| Expression pattern: |
UP |
| Associated gene: |
HuR, ELAV like RNA binding protein 1, FAT3 mRNA |
| Associated microRNA: |
miR-136-5p |
| Biological function: |
promotes lung cancer cell proliferation, invasion, growth and metastasis; stable knockdown inhibits cell proliferation and metastasis both in vitro and in vivo |
| Molecular mechanism: |
HuR binds introns flanking circ-FAT3 to promote circ-FAT3 cyclization and generation; circ-FAT3 acts as a ceRNA sponging miR-136-5p to alleviate repression of HuR mRNA, forming a positive feedback loop |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); metastasis (promotes); mRNA stability (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ-FAT3 was closely linked to larger tumor size, lymph node metastasis, later clinical stage and dismal outcome; patients with high circ-FAT3 had shorter overall and relapse-free survival time |
| Description: |
circ-FAT3 is a human FAT3-derived oncogenic circRNA that is upregulated in lung cancer and associated with larger tumor size, lymph node metastasis, advanced TNM stage, and poorer survival. It promotes lung cancer proliferation, invasion, tumor growth, and metastasis by sponging miR-136-5p to increase HuR expression, while HuR in turn enhances circ-FAT3 biogenesis by binding flanking introns, forming a positive feedback loop. |
| Confidence score: |
0.8226 |