| Expression pattern: |
UP |
| Associated gene: |
FAM83A, MEMO1, Ago2, OCT4, NANOG |
| Associated microRNA: |
miR-1253 |
| Biological function: |
Promotes NSCLC cell proliferation, stemness, and tumor growth; exosomal circ_0007385 facilitates NSCLC cell proliferation and stemness. |
| Molecular mechanism: |
Exosomal circ_0007385 acts as a sponge for miR-1253 and positively regulates FAM83A expression, thereby promoting NSCLC cell proliferation and stemness. |
| Biological pathway or process: |
proliferation (promotes); stemness (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
Exosomal circ_0007385 might be a promising application in NSCLC diagnosis and treatment. |
| Description: |
circ_0007385 is upregulated in NSCLC tissues and cells and is also delivered by NSCLC-derived exosomes. It promotes NSCLC cell proliferation, colony formation, sphere formation, stemness marker expression, and tumor growth by sponging miR-1253 and relieving repression of FAM83A. The study suggests exosomal circ_0007385 may have potential value for NSCLC diagnosis and treatment. |
| Confidence score: |
0.7977 |