| Expression pattern: |
DN |
| Associated gene: |
E-cadherin, N-cadherin, Vimentin, Slug |
| Associated microRNA: |
- |
| Biological function: |
Suppresses proliferation, migration and invasion of gastric cancer cells; inhibits EMT. |
| Molecular mechanism: |
Circ-104916 overexpression alters EMT marker expression (E-cadherin up; N-cadherin, Vimentin, Slug down), suggesting inhibition of EMT (possibly via decreasing Slug). |
| Biological pathway or process: |
EMT (inhibits); migration (inhibits); invasion (inhibits); proliferation (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; divergent primers PCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Western Blot |
| Clinical significance: |
Lower circ-104916 expression is associated with deeper invasion depth, higher tumor stage and more frequent lymphatic metastasis; potential biomarker for GC. |
| Description: |
Circ-104916 (circNEK6) is down-regulated in gastric cancer tissues and cell lines. Its overexpression suppresses gastric cancer cell proliferation, migration, and invasion, at least partly by inhibiting EMT and shifting EMT markers (E-cadherin up; N-cadherin/Vimentin/Slug down). Low circ-104916 is associated with more aggressive clinicopathological features, supporting its potential as a GC biomarker and tumor suppressor. |
| Confidence score: |
0.6537 |