| Expression pattern: |
DN |
| Associated gene: |
MCL1 |
| Associated microRNA: |
miR-622 |
| Biological function: |
Mitigates H/R-induced cardiomyocyte apoptosis and inflammatory response; improves H/R-impaired H9c2 cell viability; suppresses secretion of inflammatory factors including IL-6, TNF-alpha and IL-1beta. |
| Molecular mechanism: |
CircUBXN7 functions as a cytoplasmic miRNA sponge that targets miR-622, thereby maintaining/promoting MCL1 expression and alleviating H/R-induced apoptosis and inflammation. |
| Biological pathway or process: |
apoptosis (inhibits); inflammation (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; ELISA; Western Blot; In Vivo Animal Model |
| Clinical significance: |
Potential therapeutic target for AMI treatment and management of H/R injury. |
| Description: |
CircUBXN7 is down-regulated in AMI and H/R-treated cardiomyocytes. In H9c2 cells, circUBXN7 localizes mainly to the cytoplasm and acts through the miR-622-MCL1 axis to reduce H/R-induced apoptosis and inflammatory cytokine secretion. The study suggests circUBXN7 as a potential therapeutic target for AMI/H/R injury. |
| Confidence score: |
0.7731 |