| Expression pattern: |
UP |
| Associated gene: |
STAT1, AGO2, PCNA |
| Associated microRNA: |
miR-512-5p |
| Biological function: |
CircRPPH1 acts as an oncogene in BC and promotes proliferation, migration, colony formation, tumorigenesis and BC progression in vitro and in vivo. |
| Molecular mechanism: |
CircRPPH1 functions as a cytoplasmic ceRNA/miRNA sponge that binds miR-512-5p, relieves miR-512-5p-mediated suppression of STAT1 3′-UTR, stabilizes STAT1 mRNA, and increases STAT1 expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); ceRNA regulation (promotes); mRNA stability (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
CircRPPH1 level was associated with age and molecular subtypes, and circRPPH1 might provide a novel biomarker and potential therapeutic target of BC. |
| Description: |
CircRPPH1 is up-regulated in breast cancer tissues and cell lines and functions as an oncogenic circRNA. It is mainly cytoplasmic and promotes BC cell proliferation, migration, colony formation and in vivo tumor growth by sponging miR-512-5p, thereby stabilizing/upregulating STAT1. The study suggests circRPPH1 may serve as a potential biomarker and therapeutic target for BC. |
| Confidence score: |
0.8511 |