| Expression pattern: |
UP |
| Associated gene: |
DNMT1, DNMT1 mRNA |
| Associated microRNA: |
miR-515-5p |
| Biological function: |
circ_0040809 promotes CRC cell proliferation and migration, inhibits apoptosis, and facilitates malignant phenotypes of CRC cells. |
| Molecular mechanism: |
circ_0040809 functions as a ceRNA/miRNA sponge for miR-515-5p, thereby increasing DNMT1 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); apoptosis (inhibits); other pathway/process (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; Nuclear-Cytoplasmic Fractionation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0040809 expression is significantly associated with shorter overall survival and may be a promising prognostic biomarker and therapy target for CRC. |
| Description: |
circ_0040809 is up-regulated in colorectal cancer tissues and cell lines, and high expression is associated with shorter overall survival. Functionally, it promotes CRC cell proliferation and migration and suppresses apoptosis. Mechanistically, circ_0040809 acts as a miR-515-5p sponge to increase DNMT1 expression, supporting malignant CRC phenotypes. |
| Confidence score: |
0.7474 |