| Expression pattern: |
DN |
| Associated gene: |
HuR, human antigen R |
| Associated microRNA: |
miR-182-5p |
| Biological function: |
circHECTD1 alleviates ulcerative colitis, suppresses inflammation, reduces colonic injury, and promotes enterocyte autophagy. |
| Molecular mechanism: |
circHECTD1 acts as a miR-182-5p sponge to promote HuR expression and HuR-dependent autophagy, with involvement of AMPK/mTOR signaling, thereby reducing inflammation in UC models. |
| Biological pathway or process: |
autophagy (promotes); inflammation (inhibits); AMPK (promotes); ceRNA regulation (other); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; ELISA; Western Blot; IF (Immunofluorescence); Luciferase Reporter Assay; RNA Pull-Down; Bioinformatics Analysis; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry) |
| Clinical significance: |
circHECTD1 is decreased in colonic mucosal tissues of UC patients and has therapeutic potential for UC. |
| Description: |
circHECTD1 is down-regulated in UC colonic mucosal tissues and in experimental UC/inflammation models. Its overexpression suppresses inflammatory cytokines and NLRP3 inflammasome activation, promotes autophagy, and alleviates DSS-induced colitis via a miR-182-5p/HuR-dependent mechanism involving AMPK/mTOR signaling. |
| Confidence score: |
0.7548 |