| Expression pattern: |
UP |
| Associated gene: |
RPP25 |
| Associated microRNA: |
miR-338-3p |
| Biological function: |
circASAP1 promotes cervical cancer cell proliferation, migration, invasion, cell cycle progression, and tumor growth, while inhibiting apoptosis. |
| Molecular mechanism: |
circASAP1 functions as a sponge for miR-338-3p to upregulate RPP25 expression, thereby regulating cervical cancer cell proliferation, migration, invasion, apoptosis, and cell cycle. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Actinomycin D / DRB Stability Assay; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Western Blot; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; In Vivo Animal Model |
| Clinical significance: |
circASAP1 may be a potential biomarker for cervical cancer diagnosis. |
| Description: |
circASAP1 is upregulated in cervical cancer tissues and cells and acts as an oncogenic circRNA. It promotes proliferation, migration, invasion, cell cycle progression, and xenograft tumor growth while suppressing apoptosis, mainly by sponging miR-338-3p to increase RPP25 expression. The study proposes circASAP1 as a potential diagnostic biomarker for cervical cancer. |
| Confidence score: |
0.795 |