circRNA basic information
circBase ID: hsa_circ_0017956
Name: hsa_circ_MLLT10
Synonym: circ_0017956
Host Gene: MLLT10
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tumor samples; normal adjacent specimens; NSCLC tissues; xenograft tumors
Cell lines:

A549; H1299; NCI-H1299; BEAS-2B; HUVECs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: FOXP4, MLLT10
Associated microRNA: miR-758-3p
Biological function: promotes NSCLC cell proliferation, migration, invasion, angiogenesis and tumor growth; inhibits cell apoptosis
Molecular mechanism: circ_0017956 acts as a ceRNA/miRNA sponge for miR-758-3p to upregulate FOXP4 expression
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); ceRNA regulation (other)
Detected method:
Q
M
Validation methods:

RNase R Treatment; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis
Clinical significance:

high expression is associated with advanced TNM stage and positive lymph node metastasis; circ_0017956 siRNAs or shRNAs may be promising therapeutic molecules for NSCLC prevention
Description:

In this study, circ_0017956 was upregulated in NSCLC tissues and cells and was associated with advanced TNM stage and lymph node metastasis. Functional assays showed that circ_0017956 promotes malignant NSCLC phenotypes, including proliferation, migration, invasion, angiogenesis and in vivo tumor growth, while suppressing apoptosis. Mechanistically, cytoplasmic circ_0017956 acts as a sponge for miR-758-3p to increase FOXP4 expression, forming a circ_0017956/miR-758-3p/FOXP4 regulatory axis.
Confidence score:

0.8175
Other information
Title:

Hsa_circ_0017956 Acts as miR-758-3p Sponge to Facilitate the Progression of Non-small-Cell Lung Cancer by Regulating FOXP4 Expression.

Journal: Molecular biotechnology
Published: 2023
PubMed ID: 36763305
Study type:

combined biological and clinical study
Data availability: GSE112214; Supplementary material available at https:// doi. org/ 10. 1007/ s12033-023-00661-x
Code availability: -