| Expression pattern: |
DN |
| Associated gene: |
CDK4, Cyclin D1/CDK4 complexes |
| Associated microRNA: |
- |
| Biological function: |
circYap inhibits OSCC cell proliferation and arrests cell cycle progression, especially G1/S transition. |
| Molecular mechanism: |
circYap binds CDK4 and blocks Cyclin D1/CDK4 complex formation and nuclear translocation, thereby inhibiting G1/S transition and OSCC cell proliferation. |
| Biological pathway or process: |
proliferation (inhibits); cell cycle (inhibits); PI3K/AKT (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Transfection; BrdU; Cell Cycle Assay; Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Low circYap expression was closely correlated with TNM stage, lymph node metastasis, and decreased overall survival in OSCC; circYap may serve as a valuable therapeutic target for OSCC. |
| Description: |
circYap is downregulated in OSCC tissues and cell lines, and low expression is associated with advanced clinicopathological features and decreased overall survival. Functionally, circYap suppresses OSCC cell proliferation and arrests G1/S cell-cycle progression. Mechanistically, circYap binds CDK4, inhibits Cyclin D1/CDK4 complex formation and nuclear translocation, and thereby represses cell-cycle progression. |
| Confidence score: |
0.8369 |