| Expression pattern: |
UP |
| Associated gene: |
MMP1, Ago2 |
| Associated microRNA: |
miR-101-3p |
| Biological function: |
promotes NSCLC cell proliferation, migration and invasion; promotes tumor growth in vivo; promotes EMT |
| Molecular mechanism: |
ceRNA sponge mechanism: circ_0001859 sponges miR-101-3p to regulate MMP1 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Western Blot; Nuclear-Cytoplasmic Fractionation; CCK8; Colony Formation Assay; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Clinical Sample Validation; In Vivo Animal Model |
| Clinical significance: |
circ_0001859 expression was higher in stage III-IV than stage I-II and higher in NSCLC tissues with lymph node metastasis; proposed as a diagnostic biomarker/therapeutic target |
| Description: |
circ_0001859 is up-regulated in NSCLC tissues/cell lines and mainly localized in the cytoplasm. It promotes malignant phenotypes (proliferation, migration, invasion, EMT and tumor growth) by sponging miR-101-3p to increase MMP1 expression, forming a circ_0001859/miR-101-3p/MMP1 regulatory axis. |
| Confidence score: |
0.7094 |