| Expression pattern: |
UP |
| Associated gene: |
ALKBH5, IGF2BP1, IGF2BP2, IGF2BP3, FMRP, WWC2 |
| Associated microRNA: |
- |
| Biological function: |
promotes CRC cell growth, metastasis, and EMT in vitro and in vivo |
| Molecular mechanism: |
ALKBH5 down-regulation enhances IGF2BP2-mediated m6A modification to increase circXPO1; circXPO1 binds FMRP and promotes WWC2 mRNA decay, activating Hippo-YAP signaling |
| Biological pathway or process: |
Hippo/YAP (promotes); EMT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); m6A modification (promotes); mRNA stability (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; Colony Formation Assay; Wound Healing Assay; Transwell Assay; MeRIP / MeRIP-seq; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
high expression of circXPO1 indicated a lower survival rate of CRC patients |
| Description: |
circXPO1 (hsa_circ_0001017), derived from XPO1, is up-regulated in colorectal cancer and predicts poor survival. It is mainly cytoplasmic and promotes CRC growth, EMT, invasion and metastasis by an m6A-dependent mechanism (ALKBH5/IGF2BP2) and by binding the RBP FMRP to destabilize WWC2 mRNA, thereby activating Hippo-YAP signaling. |
| Confidence score: |
0.8912 |