circRNA basic information
circBase ID: hsa_circ_0006357
Name: hsa_circ_EZH2
Synonym: circEZH2E2-E3
Host Gene: EZH2
Genomic location(hg19): chr7:148543561-148544397:-
Genomic location(hg38): chr7:148846469-148847305:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer / PCa
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

prostate adenocarcinoma biopsy tissue samples; BPH biopsy tissue samples; xenograft tumor tissue
Cell lines:

RWPE-1; LNCaP; PC-3; DU145; 22Rv1; HUVEC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: EZH2, CDH1, DAB2IP, Ago2, H3K27me3
Associated microRNA: miR363, miR708
Biological function: promotes prostate cancer cell proliferation, migration, invasion, angiogenesis, and xenograft tumor growth
Molecular mechanism: ceRNA-like mechanism: circEZH2E2/E3 binds (sponges) miR363 and miR708, blocking their inhibitory effects on EZH2 (3′UTR and CDS), thereby upregulating EZH2 and repressing EZH2-target tumor-suppressive genes such as CDH1 and DAB2IP
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); ceRNA regulation (promotes)
Detected method:
Q
H
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; ISH (In Situ Hybridization); Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; Tube Formation Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis; Clinical Sample Validation; Cohort Study
Clinical significance:

Overexpression of circEZH2E2/E3 is significantly correlated with higher tumor grade, tumor progression, and unfavorable progression-free and disease-specific survival in PCa patients.
Description:

circEZH2E2/E3 (hsa_circ_0006357), derived from EZH2 exons 2-3, is up-regulated in prostate cancer and mainly localized in the cytoplasm. It sponges miR363 and miR708 to relieve their repression of EZH2, leading to increased EZH2/H3K27me3, repression of CDH1 and DAB2IP, and promotion of proliferation, migration/invasion, angiogenesis, and xenograft growth. High circEZH2E2/E3 is associated with tumor progression and poor progression-free and disease-specific survival.
Confidence score:

0.8841
Other information
Title:

circEZH2E2 /E3 is a dual suppressor of miR363/miR708 to promote EZH2 expression and prostate cancer progression.

Journal: Cancer science
Published: 2023
PubMed ID: 36519785
Study type:

combined biological and clinical study
Data availability: -
Code availability: -