| Expression pattern: |
UP |
| Associated gene: |
PD-L1 |
| Associated microRNA: |
miR-27b-3p |
| Biological function: |
promotes CRC cell proliferation, migration, invasion, EMT and immune escape; inhibits apoptosis; induces CD8+ T cell apoptosis/depletion |
| Molecular mechanism: |
ceRNA mechanism: circ_0089761 sponges miR-27b-3p to relieve repression of PD-L1, increasing PD-L1 expression and promoting immune escape/metastasis |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); EMT (promotes); immune regulation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Survival Analysis; ROC Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Flow Cytometry（Non-apoptosis/cycle）; TUNEL; ELISA |
| Clinical significance: |
High circ_0089761 levels were associated with tumor size, TNM staging, lower overall survival, and high diagnostic performance (AUC 0.997). |
| Description: |
circ_0089761 is up-regulated in colorectal cancer and promotes malignant phenotypes (proliferation, migration/invasion, EMT) while inhibiting apoptosis. It acts as a ceRNA by sponging miR-27b-3p to increase PD-L1, thereby enhancing immune escape by impairing CD8+ T-cell function. High circ_0089761 expression correlates with advanced stage, larger tumor size, poorer overall survival, and shows diagnostic potential. |
| Confidence score: |
0.753 |