| Expression pattern: |
UP |
| Associated gene: |
ABCC1, P-gp, LRP |
| Associated microRNA: |
miR-186-5p |
| Biological function: |
Promotes cisplatin (DDP) resistance in NSCLC; circ_0076305 knockdown improves DDP sensitivity in vitro and in vivo and suppresses tumor growth in xenografts. |
| Molecular mechanism: |
circ_0076305 functions as a ceRNA/molecular sponge for miR-186-5p to positively regulate ABCC1 expression, thereby enhancing DDP resistance. |
| Biological pathway or process: |
ceRNA regulation (promotes); drug resistance (promotes); chemoresistance (promotes); proliferation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; MTT; Western Blot; In Vivo Animal Model |
| Clinical significance: |
Potential circRNA-targeted therapy for NSCLC patients; exosomal circ_0076305 may have potential value for blood sample-based monitoring, although this was proposed for subsequent study. |
| Description: |
circ_0076305 is up-regulated in NSCLC serum exosomes, NSCLC tissues, and DDP-resistant NSCLC tissues and cells. It promotes cisplatin resistance by sponging miR-186-5p and increasing ABCC1 expression; knockdown of circ_0076305 improves DDP sensitivity in vitro and in a xenograft model. |
| Confidence score: |
0.7244 |