| Expression pattern: |
DN |
| Associated gene: |
MDM2, p53 |
| Associated microRNA: |
- |
| Biological function: |
circ-Foxo3 promotes stress-induced cancer cell apoptosis, decreases cell survival, inhibits tumor growth, and increases mouse survival in tumor models. |
| Molecular mechanism: |
circ-Foxo3 binds MDM2 and p53, enhances MDM2-mediated p53 ubiquitination and degradation, reduces Foxo3 ubiquitination by MDM2, increases Foxo3 and Puma levels, and thereby promotes apoptosis. |
| Biological pathway or process: |
apoptosis (promotes); proliferation (inhibits); ubiquitination (other); p53 signaling (other) |
| Detected method: |
Q
B
|
| Validation methods: |
RT-qPCR; Northern Blot; Clinical Sample Validation; Transfection; Annexin V/PI Flow Cytometry; Cell Cycle Assay; In Vivo Animal Model; H&E Staining; TUNEL; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; ISH (In Situ Hybridization); IHC (Immunohistochemistry); Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
circ-Foxo3 is expressed at significantly lower levels in breast carcinoma biopsies than in benign tissues and may be developed as an agent for cancer intervention. |
| Description: |
circ-Foxo3 is down-regulated in breast carcinoma biopsies and cancer cell lines but increases during apoptosis. Functionally, circ-Foxo3 promotes stress-induced apoptosis and suppresses tumor growth by binding MDM2 and p53, enhancing p53 ubiquitination while protecting Foxo3 from MDM2-mediated degradation, leading to increased Puma expression. |
| Confidence score: |
0.8229 |