| Expression pattern: |
DN |
| Associated gene: |
LATS1, YAP, p-YAP, AGO2 |
| Associated microRNA: |
miR-424 / has-miR-424-5p |
| Biological function: |
circLARP4 functions as a tumor suppressive factor in gastric cancer cells, inhibiting DNA synthesis, cell proliferation, colony formation and invasion. |
| Molecular mechanism: |
circLARP4 acts as a ceRNA/miRNA sponge for miR-424, thereby regulating LATS1 and downstream YAP/p-YAP signaling in gastric cancer cells. |
| Biological pathway or process: |
Hippo/YAP (inhibits); proliferation (inhibits); invasion (inhibits); ceRNA regulation (promotes); drug response (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Colony Formation Assay; Transwell Assay; Western Blot; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circLARP4 was downregulated in GC tissues, negatively associated with tumor size and lymphatic metastasis, predicted longer overall survival when highly expressed, and acted as an independent prognostic factor for OS; high expression was also associated with more favorable therapeutic outcome after oxaliplatin and 5-Fu adjuvant chemotherapy. |
| Description: |
circLARP4 is a human LARP4-derived circRNA that is downregulated in gastric cancer tissues and cell lines. It mainly localizes in the cytoplasm and suppresses GC cell proliferation and invasion by sponging miR-424 and regulating the LATS1/YAP pathway. Clinically, higher circLARP4 expression is associated with longer overall survival and more favorable therapeutic outcome after oxaliplatin and 5-Fu chemotherapy. |
| Confidence score: |
0.874 |